Composite

Part:BBa_K4898003:Design

Designed by: Aayushi Agrawal   Group: iGEM23_IIT-Delhi   (2023-10-12)


Inducible dual promoter system to produce AlnA under Ptac and rhlAB under Ptet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1353
    Illegal BamHI site found at 1913
    Illegal XhoI site found at 2089
    Illegal XhoI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2268
    Illegal NgoMIV site found at 2989
    Illegal NgoMIV site found at 3102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1578
    Illegal BsaI site found at 2618
    Illegal BsaI.rc site found at 1762


Design Notes

None


Source

AlnA comes from Acinetobacter radioresistens KA53 and rhlAB comes from Pseudomonas aeruginosa

References